通過(guò)銅催化的疊氮化物-炔烴環(huán)加成反應(yīng)（SPAA）和應(yīng)變促進(jìn)的疊氮化物-炔烴環(huán)加成反應(yīng)（點(diǎn)擊化學(xué)反應(yīng)）制備了具有共價(jià)連接的熒光染料分子的磷酸鈣納米粒子。比較并量化了兩次點(diǎn)擊反應(yīng)的反應(yīng)動(dòng)力學(xué)和標(biāo)記度。對(duì)于氧化敏感性分子，由于反應(yīng)條件較溫和且不存在銅（II），因此首選SPAAC方法。高熒光AF-488標(biāo)記的納米顆粒用于超分辨率熒光，即隨機(jī)光學(xué)重建顯微鏡（STORM）。通過(guò)這種方法，在細(xì)胞以20–30 nm的分辨率攝取后，在HeLa細(xì)胞內(nèi)部的溶酶體中觀察到了單個(gè)納米顆粒（直徑約60 nm）。

Synthesis and intracellular tracing surface-functionalized calcium phosphate nanoparticles by super-resolution microscopy (STORM)

Abstract Calcium phosphate nanoparticles with covalently attached fluorescent dye molecules were prepared by copper-catalysed azide-alkyne cycloaddition (CuAAC) and by strain-promoted azide-alkyne cycloaddition (SPAAC), i.e. click chemistry. The reaction kinetics and labeling degrees of the two click reactions were compared and quantified. For oxidation-sensitive molecules, the SPAAC method is preferable due to the milder reaction conditions and the absence of copper(II). The highly fluorescent AF-488-labeled nanoparticles were applied for super-resolution fluorescence microscopy, i.e. stochastic optical reconstruction microscopy (STORM). By this method, individual nanoparticles (diameter about 60 nm) were observed in endolysosomes inside HeLa cells after cellular uptake at a resolution of 20–30 nm.

原文鏈接：超分辨顯微鏡（STORM）合成和細(xì)胞內(nèi)示蹤表面功能化磷酸鈣納米顆粒 - ScienceDirect