最新日本超大胆视频网站_国产成人综合亚洲av_免费播放欧美黄色大片频_成年在线观看免费网站_国产精品中文字幕欧美专区日韩_國語對白嫩模_无毒不卡在线观看无需下载_911精品亚洲永久免费精品_欧美高清国产在线有码_曰韩无码二三区中文字幕

193-9003-8897
廣州微視光學(xué)科技有限公司

超分辨顯微鏡(STORM)合成和細(xì)胞內(nèi)示蹤表面功能化磷酸鈣納米顆粒

瀏覽:183 時(shí)間:2023-09-05
通過(guò)銅催化的疊氮化物-炔烴環(huán)加成反應(yīng)SPAA和應(yīng)變促進(jìn)的疊氮化物-炔烴環(huán)加成反應(yīng)點(diǎn)擊化學(xué)反應(yīng)制備了具有共價(jià)連接的熒光染料分子的磷酸鈣納米粒子

通過(guò)銅催化的疊氮化物-炔烴環(huán)加成反應(yīng)(SPAA)和應(yīng)變促進(jìn)的疊氮化物-炔烴環(huán)加成反應(yīng)(點(diǎn)擊化學(xué)反應(yīng))制備了具有共價(jià)連接的熒光染料分子的磷酸鈣納米粒子。比較并量化了兩次點(diǎn)擊反應(yīng)的反應(yīng)動(dòng)力學(xué)和標(biāo)記度。對(duì)于氧化敏感性分子,由于反應(yīng)條件較溫和且不存在銅(II),因此首選SPAAC方法。高熒光AF-488標(biāo)記的納米顆粒用于超分辨率熒光顯微鏡,即隨機(jī)光學(xué)重建顯微鏡(STORM)。通過(guò)這種方法,在細(xì)胞以20–30 nm的分辨率攝取后,在HeLa細(xì)胞內(nèi)部的溶酶體中觀察到了單個(gè)納米顆粒(直徑約60 nm)。


55e8ae81a17e6a23e983082a9f86691


Synthesis and intracellular tracing surface-functionalized calcium phosphate nanoparticles by super-resolution microscopy (STORM)

Abstract Calcium phosphate nanoparticles with covalently attached fluorescent dye molecules were prepared by copper-catalysed azide-alkyne cycloaddition (CuAAC) and by strain-promoted azide-alkyne cycloaddition (SPAAC), i.e. click chemistry. The reaction kinetics and labeling degrees of the two click reactions were compared and quantified. For oxidation-sensitive molecules, the SPAAC method is preferable due to the milder reaction conditions and the absence of copper(II). The highly fluorescent AF-488-labeled nanoparticles were applied for super-resolution fluorescence microscopy, i.e. stochastic optical reconstruction microscopy (STORM). By this method, individual nanoparticles (diameter about 60 nm) were observed in endolysosomes inside HeLa cells after cellular uptake at a resolution of 20–30 nm.


原文鏈接:超分辨顯微鏡(STORM)合成和細(xì)胞內(nèi)示蹤表面功能化磷酸鈣納米顆粒 - ScienceDirect

1顯示全部